V2258
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 | Immunofluorescence Imna(-/-) mouse embryonic fibroblasts stained with Monoclonal Anti-Vimentin, clone LN-6 (Cat. No. V2258) at a 1:40 dilution (red), Anti-Actin (blue) and DAPI (yellow). From Shyam Khatau, Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD. |
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V2258
Sigma
Monoclonal Anti-Vimentin antibody produced in mouse
clone LN-6, ascites fluid
| MDL number: | MFCD00162904 |
Description
| Immunogen | human thymic nuclear extract |
| Specificity | Recognizes an epitope of the cytoskeleton protein vimentin that is not detected in human lymphoid cells or derived malignancies. It is therefore useful in differentiating sarcomas from lymphomas. |
Properties
| antibody form | ascites fluid |
| clone | LN-6 (monoclonal) |
| contains | 15 mM sodium azide |
| species reactivity | feline, bovine, pig, rat, rabbit, human, mouse, sheep |
| application(s) | immunoblotting: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections | |
| immunoprecipitation: suitable | |
| indirect immunofluorescence: suitable using cultured cells | |
| isotype | IgM |
| Gene Information | human ... VIM(7431) |
| rat ... Vim(81818) | |
| mouse ... Vim(22352) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Stathopoulos, E., et al. J. Histochem. Cytochem. 37, 1363, (1989) Abstract |
| Zhu, H., et al., Lsh, a modulator of CpG methylation, is crucial for normal histone methylation EMBO J. 22, 5154-5162, (2003) Abstract | |
| Cho, E., et al., Association of Lsh, a Regulator of DNA Methylation, with Pericentromeric Heterochromatin Is Dependent on Intact Heterochromatin Mol. Cell. Biol. 23, 8416-8428, (2003) Abstract | |
| Michel, B.A., et al., Expression and Regulation of Toll-Like Receptor 2 in Rheumatoid Arthritis Synovium Am. J. Pathol. 162, 1221-1227, (2003) Abstract |
